Effect of Allopurinol (4-Hydroxypyrazolo[3,4-d]pyrimidine) on Xanthine Accumulation by Milk Xanthine Oxidase in vitro

Inhibitory Effect of Ruta graveolens L. Extract on Guinea Pig Liver and Bovine Milk Xanthine Oxidase

      Flavonoids could serve as potent inhibitors of xanthine oxidase (XO). In the present study, the effects of Ruta graveolens L. extract and its major isolated flavonoids, quercetin and rutin, on guinea pig liver XO have been investigated. The inhibitory effects of R. graveolens, quercetin and its glycoside form, rutin, were assayed spectrophotometrically. R. graveolens...

Inhibition of xanthine oxidase by allopurinol: its lack of effect on models of inflammation.

and 'lupus like' disease: association with anti-phospholipid antibodies. Leucopenia after gold and sulphasalazine treatment Sir: I read the interesting report by Bliddal and colleagues of three patients who developed leucopenia after both gold and sulphasalazine treatment.' HLA-DR3 as a marker for toxic reactions after treatment with gold or D-penicillamine could not be shown. Patient 1, howeve...

The composition of milk xanthine oxidase.

The composition of milk xanthine oxidase has been reinvestigated. When the enzyme is prepared by methods that include a selective denaturation step in the presence of sodium salicylate the product is obtained very conveniently and in high yield, and is homogeneous in the ultracentrifuge and in recycling gel filtration. It has specific activity higher than previously reported preparations of the...

Xanthine oxidase inhibition by allopurinol increases in vitro pyrazinamide-induced hepatotoxicity in HepG2 cells.

Despite the important role of pyrazinamide in tuberculosis treatment, little is known about the mechanism of pyrazinamide-induced hepatotoxicity. We inhibited xanthine oxidase in HepG2 cells by using a nontoxic concentration of allopurinol, a well-known xanthine-oxidase inhibitor. This increased in vitro pyrazinamide toxicity in HepG2 cells, which suggests that the hydroxy metabolites of pyrazi...

Steps in the oxidation of xanthine to uric acid catalysed by milk xanthine oxidase.